Reporter and Recombinase Expressing Mice
Typical reporter constructs in GM mice express the Green Fluorescent Protein (GFP) or bacterial beta-galactosidase (LacZ) gene under specific promoters.
Cre recombinase is the most commonly used recombinase at present. It catalyzes the recombination between loxP sites. Other recombinases (Flp, Dre, phiC31, etc.) and various inducible forms of recombinases have been and are being developed, FLP recombinase with Frt target sequences being currently perhaps the most commonly used non-cre recombinase. Both loxP and Frt sites are included in EUCOMM alleles.
The Mouse genome Informatics (MGI) Cre portal contains a collection of data on expression and activity for recombinase-containing transgenes and knock-in alleles.
The CREATE (Coordination of resources for conditional expression of mutated mouse alleles) project aims to develop a strategy for the integration and dissemination of Cre driver strains for modelling aspects of complex human diseases in the mouse.
The MouseCre initiative adds to the existing CRE-X-Mice database at MSHRI (Toronto, Canada), the CRE database at MGI (The Jackson Laboratory, Bar Habor, Maine, USA) and the CreZOO database at the Fleming Institute (Vari, Greece). All these different CRE databases and resources are coordinated through the CREATE consortium, a CRE recombinase portal organized by EBI (Hinxton-Cambridge, UK).
The Institut Clinique de la Souris (ICS) has developed a specific CreERT2 resource that is now available to the scientific community: The Mouse Cre and CreERT2 zoo.